bio rad chef mapper pfge system  (Bio-Rad)

Journal: Journal of hazardous materials

Article Title: A newly developed Escherichia coli isolate panel from a cross section of U.S. animal production systems reveals geographic and commodity-based differences in antibiotic resistance gene carriage.

doi: 10.1016/j.jhazmat.2019.120991

Figure Lengend Snippet: Fig. 2. Distribution and co-occurrence of isolates by commodity based on Pulsed Field Gel Electrophoresis (PFGE) groupings.

Article Snippet: Digested DNA was separated using the Bio-Rad CHEF-DRII PFGE system (Bio-Rad, Hercules, CA) per manufacturer’s instructions.

Techniques: Pulsed-Field Gel, Electrophoresis

Journal: Foodborne Pathogens and Disease

Article Title: Antibiotic Resistance Profiling, Analysis of Virulence Aspects and Molecular Genotyping of Staphylococcus aureus Isolated in Sicily, Italy

doi: 10.1089/fpd.2017.2338

Figure Lengend Snippet: Staphylococcus aureus Strains Isolated in Sicily Analyzed by Multilocus Sequence Typing and Pulsed-Field Gel Electrophoresis

Article Snippet: The DNA was digested with 20 U Sma I (New England Biolabs) at room temperature for 4 h. Macrorestriction fragments were separated using a BioRad CHEF System (30" 12 h, 15" 6 h, 1% gel in Tris-borate-EDTA [TBE] 0.5 × , 200 V) and a PFGE size standard (CHEF DNA Size Standard, Lambda [λ] ladder; BioRad) was added.

Techniques: Isolation, Sequencing, Pulsed-Field Gel

Journal: Foodborne Pathogens and Disease

Article Title: Antibiotic Resistance Profiling, Analysis of Virulence Aspects and Molecular Genotyping of Staphylococcus aureus Isolated in Sicily, Italy

doi: 10.1089/fpd.2017.2338

Figure Lengend Snippet: Pulsotypes of Staphylococcus aureus isolates. (A) Example of the PFGE profiles after SmaI digestion of genomic DNA, detected in this study. (B) Dendrogram of the ten PFGE profiles.

Article Snippet: The DNA was digested with 20 U Sma I (New England Biolabs) at room temperature for 4 h. Macrorestriction fragments were separated using a BioRad CHEF System (30" 12 h, 15" 6 h, 1% gel in Tris-borate-EDTA [TBE] 0.5 × , 200 V) and a PFGE size standard (CHEF DNA Size Standard, Lambda [λ] ladder; BioRad) was added.

Techniques:

Journal: BMC Genomics

Article Title: The expansion of heterochromatin blocks in rye reflects the co-amplification of tandem repeats and adjacent transposable elements

doi: 10.1186/s12864-016-2667-5

Figure Lengend Snippet: PFGE separation of Pst I-restricted BAC inserts harboring pSc200 arrays. a Ethidium bromide stained gel, b Southern hybridization probed with pSc200. Lane 1: BAC clone 126C20, lane 2: 119C15, lane 3: 119 M22, lane 4: 114I10

Article Snippet: The agarose plugs containing the DNA were loaded into a CHEF-DRIII PFGE system device (Bio-Rad) for PFGE through a 1 % agarose gel.

Techniques: Staining, Hybridization

Journal: BMC Veterinary Research

Article Title: Faecal shedding and strain diversity of Listeria monocytogenes in healthy ruminants and swine in Northern Spain

doi: 10.1186/1746-6148-5-2

Figure Lengend Snippet: Dendrogram (UPGMA) of the Apa I PFGE patterns of L. monocytogenes isolates from 6 different herds (A-G as in Table 2) . The isolates have been coded according to sample type of origin: P, pool of faeces; I, individual faecal sample.

Article Snippet: Fragments were separated by electrophoresis in a CHEF-DRII system (BioRad) at a constant temperature of 14°C during 5 h using an initial switch time of 15 s and a final switch time of 35 s and a further 15 h with switch times of 2–20 s. Gels were normalised by alignment with the Lambda Ladder PFGE size standard (New England Biolabs, MA, USA) and Salmonella Braenderup control strain H9812 digested with Xba I [ ].

Techniques: